| CatalogCode: | NB100-65902 |
| ProductName: | CD34 Antibody |
| Product Description: | Rat Monoclonal anti-CD34 (MEC14.7) |
| Clone: | MEC14.7 |
| Clonality: | Monoclonal |
| Specificity: | NB100-65902 recognises the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies. |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.25 mg protein G purified Rat ascites. |
| Uses: | This antibody is useful for immunoprecipitation, flow cytometry / FACS analysis |
| Localization: | Type I membrane protein. |
| Background: | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2a |
| Host_Name: | Rat |
| Buffer: | Phosphate buffered saline pH7.4 |
| ListPrice: | 285 |
| AppSummary: | FACS, IP |
| SpeciesSummary: | Mu |
| ALTnames: | anti-Cluster designation 34 antibody, anti-Hematopoietic progenitor cell antigen CD34 antibody, anti-HPCA1 antibody |
| ProteinTarget: | CD34 |
| PackageSize: | 0.25 mg |
| GeneralRef: | 1. Garlanda, C. et al. (1997) Characterization of MEC14.7, a new monoclonal antibody recognising mouse CD34: a useful reagent for identifying and characterizing blood vessels and haematopoietic precursors. Eur. J. Cell Biol. 73: 368-377. 2. Winding, B. et al. (2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice. Clin. Cancer Res. 8: 1932-1939. |
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