| CatalogCode: | NB100-65792 |
| ProductName: | CD8 ALPHA Antibody |
| Product Description: | Mouse Monoclonal anti-CD8 ALPHA (OX-8) |
| Clone: | OX-8 |
| Clonality: | Monoclonal |
| Immunogen: | Rat thymocyte membrane glycoproteins. |
| Specificity: | NB100-65792 recognises the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.* This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (3).Clone MRC OX-8 is suitable for use in in vitro blocking studies. AbD Serotec recommend the use of MCA48XZ for this purpose (5).This product is routinely tested in flow cytometry on rat splenocytes. |
| CrossReactivity: | Rat. Not yet tested in other species. |
| Packaging: | 0.25 mg protein G purified Mouse IgG. |
| Uses: | This antibody is useful for Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, flow cytometry / FACS analysis |
| Localization: | Isoform 1: Cell membrane; single pass type I membrane protein. Isoform 2: Secreted. |
| Background: | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline pH7.2 |
| ListPrice: | 285 |
| AppSummary: | FACS, IHC-P, IHC-Fr |
| SpeciesSummary: | Rt |
| ALTnames: | anti-CD 8a antibody, anti-CD8 alpha chain antibody, anti-CD8 antibody, anti-CD8 antigen alpha chain antibody, anti-CD8 antigen alpha polypeptide (p32) antibody, anti-CD8a antibody, anti-CD8a antigen antibody, anti-CD8a molecule antibody, anti-Leu 2 antibody, anti-Leu2 antibody, anti-Leu2 T lymphocyte antigen antibody, anti-MAL antibody, anti-OKT8 T cell antigen antibody, anti-p32 antibody, anti-T cell antigen Leu2 antibody, anti-T cell co receptor antibody, anti-T cell coreceptor antibody, anti-T cell surface glycoprotein CD8 alpha chain antibody, anti-T lymphocyte differentiation antigen T8/Leu 2 antibody, anti-T lymphocyte differentiation antigen T8/Leu2 antibody, anti-T8 T cell antigen antibody |
| ProteinTarget: | CD8 ALPHA |
| PackageSize: | 0.25 mg |
| GeneralRef: | 1. Brideau, R. J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur. J. Immunol. 10: 609-615. 2. Lyscom, N. and Brueton, M. J. (1982) Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies. Immunology 45: 775-783. 3. Whiteland, J. L. et al. (1995) Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320. 4. Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. Immunology. 52: 631-640. 5. Popov, I. et al. (2001) The effect of an Anti-HLA-B27 Immune Response on CTL Recognition of Chlamydia. J. Immunol. 167: 3375-3382. 6. Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression*. Eur. J. Immunol. 22: 2841-2848. 7. Mitnacht, R. et al. (1998) Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J. Immunol. 160: 700-707. 8. Thomas, M. L. and Green, J. R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens. Eur. J. Immunol. 13: 855-858. |
| NotesMain: | Immunohistology: This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. |
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