| CatalogCode: | | NB100-65581 |
| ProductName: | | FYN Antibody |
| Product Description: | | Mouse Monoclonal anti-FYN (FYN-01) |
| Clone: | | FYN-01 |
| Clonality: | | Monoclonal |
| Immunogen: | | Bacterially expressed recombinant fragment of human fyn aa 7-176. |
| Specificity: | | NB100-65581 recognizes p59fyn (fyn), a 59kDa member of the src family of non receptor protein tyrosine kinases, expressed by T cells and Natural Killer (NK) cells and highly expressed in brain.Fyn plays a vital role in thymocyte development and T cell activation and is essential for TCR-mediated signal transduction through association with the T Cell Receptor (TCR) eta chain/CD3 Complex. This association, results in protein kinase activation and the subsequent protein tyrosine phosphorylation of multiple immunoreceptor tyrosine-based activation motifs (ITAMs), which triggers the phosphorylation of further substrates downstream in the T cell signaling cascade.Recent studies into Alzheimer's Disease have implicated fyn in neurodegeneration, through the tyrosine phosphorylation of the microtubule-associated protein tau, a prominent component of neurofibrillary tangles.*NB100-65581 detects a band of approximately 59kDa in Mouse EL-4 cell lysates in Western Blotting. |
| CrossReactivity: | | Human. Not yet tested in other species. |
| Packaging: | | 0.1 mg protein A purified Mouse ascites. |
| Uses: | | This antibody is useful for Immunohistochemistry-Frozen, Western Blot, immunoprecipitation |
| Localization: | | Cytoplasmic |
| Control: | | Brain |
| Background: | | Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1. The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | | protein A purified |
| Isotype: | | IgG |
| Host_Name: | | Mouse |
| Buffer: | | Phosphate buffered saline pH7.4 |
| ListPrice: | | 285 |
| AppSummary: | | IHC-Fr, WB, IP |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-C syn protooncogene antibody, anti-FYN oncogene related to SRC FGR YES antibody, anti-MGC45350 antibody, anti-OKT3 induced calcium influx regulator antibody, anti-P59 FYN antibody, anti-Protein tyrosine kinase fyn antibody, anti-Proto oncogene tyrosine protein kinase fyn antibody, anti-Protooncogene Syn antibody, anti-SLK antibody, anti-Src like kinase antibody, anti-Src yes related novel gene antibody, anti-Src/yes related novel gene antibody, anti-SYN antibody, anti-Tyrosine kinase p59fyn T antibody, anti-Tyrosine kinase p59fyn(T) antibody |
| ProteinTarget: | | FYN |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | 1. Ho, G.J. et al. (2005) Altered p59Fyn kinase expression accompanies disease progression in Alzheimer's disease: implications for its functional role. Neurobiol. Aging. 26(5):625-635. 2. Lee, G. et al. (2004) Phosphorylation of Tau by Fyn: Implications for Alzheimer's disease. J. Neuroscience 24(9):2304-2312. 3. Olszowy, M.W. et al. (1995) Comparison of p56lck and p59fyn protein expression in thymocyte subsets, peripheral T cells, NK cells and lymphoid cell lines. J. Immunol. (1995). 155(9):4236-4240. 4. Zamoyska, R. et al. (2003) The influence of the src-family kinases Lck and Fyn, on T cell differentiation, survival and activation. Immunol. Rev. 191:107-118. |
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