| CatalogCode: | NB100-65563 |
| ProductName: | CD34 CLASS II Antibody |
| Product Description: | Mouse Monoclonal anti-CD34 CLASS II (QBEND/10) |
| Clone: | QBEND/10 |
| Clonality: | Monoclonal |
| Immunogen: | Human endothelial cell membrane vesicles |
| Specificity: | NB100-65563 recognises the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane.QBEND-10 immunoprecipitates a glycoprotein of Mr 110kD expressed on haemopoietic cells and on the established myeloid leukemic cell line KG1a. QBEND-10 recognises the Class II epitope on CD34. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.2 mg protein A purified Mouse ascites. |
| Uses: | This antibody is useful for Immunohistochemistry-Frozen, Western Blot, Immunohistochemistry-Paraffin, immunoprecipitation, flow cytometry / FACS analysis |
| Localization: | Type I membrane protein. |
| Background: | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 295 |
| AppSummary: | IHC-Fr, WB, IHC-P, IP, FACS |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CD34 antibody, anti-Cluster designation 34 antibody, anti-Hematopoietic progenitor cell antigen CD34 antibody, anti-HPCA1 antibody |
| ProteinTarget: | CD34 CLASS II (NOT FOR SALE IN USA) |
| PackageSize: | 0.2 mg |
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