| CatalogCode: | NB100-65390 |
| ProductName: | CD62L Antibody |
| Product Description: | Rat Monoclonal anti-CD62L (MEL-14) |
| Clone: | MEL-14 |
| Clonality: | Monoclonal |
| Immunogen: | Mouse B cell lymphoma, 38C-14 |
| Specificity: | NB100-65390 reacts with a 90kD protein involved in the homing of lymphocytes to peripheral lymph nodes (CD62L or L-selectin) L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils (1). Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (2). |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.25 mg protein G purified Rat ascites. |
| Uses: | This antibody is useful for Immunohistochemistry-Frozen, immunoprecipitation, flow cytometry / FACS analysis |
| Background: | The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. Human CD62L (L Selectin) is comprised of an aminoterminal C type lectin binding domain, an epidermal growth factor like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells. It is expressed on bone marrow myeloid progenitor cells, erythroid precursor cells and some thymocytes. It is preferentially localized to the tips of lymphocytes and polymorphonuclear cells microvilli. Bioactive soluble L Selectin derived from lymphocytes and neutrophils is present in normal and pathological biological fluids. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2a |
| Host_Name: | Rat |
| Buffer: | Phosphate buffered saline pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | FACS, IP, IHC-Fr |
| SpeciesSummary: | Mu |
| ALTnames: | anti-gp90 MEL antibody, anti-hLHRc antibody, anti-L Selectin antibody, anti-LAM1 antibody, anti-LECAM1 antibody, anti-Leukocyte adhesion molecule 1 antibody, anti-Leukocyte surface antigen Leu8 antibody, anti-LNHR antibody, anti-LSEL antibody, anti-LYAM1 antibody, anti-Lymph node homing receptor antibody, anti-Lymphocyte adhesion molecule 1 antibody, anti-PLNHR antibody, anti-Selectin L antibody, anti-SELL antibody, anti-TQ1 antibody |
| ProteinTarget: | CD62L |
| PackageSize: | 0.25 mg |
| GeneralRef: | 1. Fink, P. et al. (1985) Homing receptor bearing thymocytes, an immunocomponent cortical subpopulation. Nature 313: 233-235. 2. Gallatin, W.M. et al. (1983) A cell surface molecule involved in organ specific homing of lymphocytes. Nature 304: 30-34. 3. Lewinsohn, D.M. et al. (1987) Leucocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes and other leucocytes. J. Immunol. 138: 4313-4321. 4. Reichert, R.M. et al. (1984) A homing receptor-bearing cortical thymocyte subset: Implications for thymus cell migration and the nature of cortisone-resistant thymocytes. Cell 38: 89-99. 5. Siegleman, M. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface receptor Ly-22: Role of the EGF domain in endothelial binding. Cell 61: 611-622. 6. Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to epitopes of an 85 -95kD glycoprotein antigen differentially inhibits lymphocyte binding to lymph node, mucosal or synovial end cells. J. Cell. Biol. 105: 983 - 990. |
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