| CatalogCode: | | NB100-65361 |
| ProductName: | | CD45 Antibody |
| Product Description: | | Mouse Monoclonal anti-CD45 (HIS41) |
| Clone: | | HIS41 |
| Clonality: | | Monoclonal |
| Immunogen: | | PVG RT7.2 +ve rat thymocytes. |
| Specificity: | | NB100-65361 recognises the rat RT7.2 alloantigen - it does not react with RT7.1. RT7.2 is expressed on the surface of all peripheral leucocytes, as well as on bone marrow cells of lymphoid and myeloid lineage. Immunoprecipitation studies demonstrate a band of 180kD from thymocytes, and 190-220kD from T and B cells. Sequential immunoprecipitation studies have revealed that HIS41 recognises a determinant of the leucocyte common antigen (LCA, CD45). |
| CrossReactivity: | | Rat. Not yet tested in other species. |
| Packaging: | | 2 ml of Tissue Culture Supernatant. |
| Uses: | | This antibody is useful for Immunohistochemistry-Frozen, immunoprecipitation, flow cytometry / FACS analysis |
| Localization: | | Type I membrane protein. |
| Background: | | CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | | Tissue culture supernatant |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| ListPrice: | | 285 |
| AppSummary: | | FACS, IP, IHC-Fr |
| SpeciesSummary: | | Rt |
| ALTnames: | | anti-B220 antibody, anti-CD 45 antibody, anti-CD45 antigen antibody, anti-GP180 antibody, anti-L CA antibody, anti-LCA antibody, anti-Leukocyte common antigen antibody, anti-LY5 antibody, anti-Protein tyrosine phosphatase receptor type C antibody, anti-Protein tyrosine phosphatase receptor type c polypeptide antibody, anti-PTPRC antibody, anti-SCID due to PTPRC deficiency antibody, anti-T200 antibody, anti-T200 glycoprotein antibody |
| ProteinTarget: | | CD45 (RT7.2) |
| PackageSize: | | 2 ml |
| GeneralRef: | | 1. Butcher, G.W. et al. (1987) A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenetics. 14; 163 - 176. 2. Kampinga, J. et al. (1990) Differences in turnover between thymic medullary dendritic cells and a subset of cartical macrophages. J. Immunol. 145: 1659 - 1663. 3. Havenith, C. E. G. et al. (1993) Migration of Dendritic Cells into the Draining Lymph Nodes of the Lung after Intracheal Installation. Am. J. Respir. Cell. Mol. Biol. 9: 484 - 488. 4. Tschernig, T. et al. (1997) The Lung as a source and a Target Organ for T- and B- lymphocytes. Am. J. Respir. Cell. Mol. Biol. 17: 414 - 421. 5. Løvas, K. et al. (1996) Sequestration patterns of transfused rat neutrophilic granlocytes under normal and inflammatory conditions. Eur. J. Haematol. 50: 221 - 229. |
| NotesMain: | | Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Method sheets are available on request |
| more information: | | company product webpage |
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