| CatalogCode: | NB100-64980 |
| ProductName: | CD138 Antibody |
| Product Description: | Mouse Monoclonal anti-CD138 (B-A38) |
| Clone: | B-A38 |
| Clonality: | Monoclonal |
| Immunogen: | U266 cell line |
| Specificity: | NB100-64980 recognises a heparan sulfate rich membrane glycoprotein of 200kD known as Syndecan-1 (CD138). Syndecan functions as a receptor for collagen, fibronectin and thrombospondin.NB100-64980 stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Immunohistochemistry-Frozen, flow cytometry / FACS analysis, Immunohistochemistry-Paraffin |
| Localization: | Membrane; Single-pass type I membrane protein. |
| Control: | Bone Marrow |
| Background: | CD138 / Syndecan 1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 285 |
| AppSummary: | IHC-Fr, FACS, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Syndecan antibody, anti-CD 138 antibody, anti-CD138 antibody, anti-CD138 antigen antibody, anti-Heparan sulfate proteoglycan fibroblast growth factor receptor antibody, anti-SDC 1 antibody, anti-SDC antibody, anti-SDC1 antibody, anti-SYND 1 antibody, anti-SYND1 antibody, anti-Syndecan 1 antibody, anti-syndecan1 antibody, anti-Syndecan1 precursor antibody |
| ProteinTarget: | CD138 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Borset, M. et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation. Br. J. Haem. 85: 446. 2. Anttonen, A. et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma. Brit. J. Cancer 79: 558 - 564. |
| NotesMain: | Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request. Immunohistology: This product requires heat pre-treatment. Sodium citrate buffer pH6.0 is recommended for this purpose. |
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