| CatalogCode: | NB100-648 |
| ProductName: | VEGF Antibody |
| Product Description: | Mouse Monoclonal anti-VEGF (VG76e) |
| Clone: | VG76e |
| Clonality: | Monoclonal |
| Immunogen: | Human VEGF189 expressed in E.coli |
| Specificity: | This antibody is specific for VEGF. |
| CrossReactivity: | Cross-reacts with Human, Cow and Pig. Not yet tested in other species. |
| Packaging: | 0.1 mg Affinity purified Mouse ascites. |
| Uses: | This antibody has been useful for Western blot, Immunoprecipitation, ELISA, and Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Detects a band between 38-44 kDa.Good results have been reported on human, bovine and porcine paraffin sections, but only after microwave treatment and using Tris HCl pH 10 buffer (NOT citrate buffer). VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each. |
| Localization: | This antibody is highly specific, but only weakly stains transfected cells (5-10%). |
| Background: | Vascular endothelial growth factor (VEGF) was recently identified as a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparently arising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 are potent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1 induces apoptosis; VEGF protects endothelial cells from apoptosis. We found that TGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGF synthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogates TGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates an unexpected role of VEGF and indicates that VEGF can be pharmacologically converted into an apoptotic factor, a novel approach to antiangiogenesis therapy (2). |
| ProductRef: | 1. Zhang L, et al. Validation of anti-vascular endothelial growth factor (anti-VEGF) antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium. J Pathol 185:402-8 (1998). 2. Mannheim, D., et al. (2007) Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularization, 01215.02006. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | ELISA, IP, WB, IHC-P |
| SpeciesSummary: | Bv, Hu, Po |
| ALTnames: | anti-Vascular Endothelial Growth Factor antibody, anti-Vascular Permeability Factor antibody, anti-VEGFA antibody, anti-VPF antibody |
| ProteinTarget: | VEGF antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Houck KA, et al. The vascular endothelial growth factor family: identification of a fourth molecular species and characterization of alternative splicing of RNA . Mol Endocrinol 5(12):1806-14, 1991. 2. Ferrari G, et al. VEGF, a prosurvival factor, acts in concert with TGF-beta 1 to induce endothelial cell apoptosis. Proc Natl Acad Sci USA 103(46):17260-5, 2006. |
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