| CatalogCode: | NB100-646 |
| ProductName: | SOCS3 Antibody |
| Product Description: | Rabbit Polyclonal anti-SOCS3 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (QLPGPIREFLDQYDAPL) corresponding to amino acids 209-225 of the human/rat/mouse SOCS 3. |
| Specificity: | Detects a band at 27kDa. |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.025 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used for ELISA, IP, and Western Blot. By Western blot, this antibody detects a band of approximately 27 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 3. Samples wereboiled prior to subject 10% SDS-polyacrylamide gelelectrophoresis. |
| Background: | Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling) (1-3). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box (4). The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism (5). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH (6-8). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 325 |
| AppSummary: | ELISA, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-CIS3 antibody, anti-Cish3 antibody, anti-Cytokine induced SH2 protein 3 antibody, anti-E2a Pbx1 target gene in fibroblasts 10 antibody, anti-EF 10 antibody, anti-SSI3 antibody, anti-STAT induced STAT inhibitor 3 antibody, anti-Suppressor of cytokine signaling 3 antibody |
| ProteinTarget: | SOCS 3 antibody |
| PackageSize: | 0.025 mg |
| GeneralRef: | 1. Gregorieff A et al. (2000) J. Biol. Chem. 275(28): 21596-21604. 2. Krebs DL and Hilton DJ (2000) J. Cell Sci. 113:2813-2819 3. Ram PA and Waxman (1999) J. Biol. Chem. 274(50): 35553-61. 4. Hilton DJ et al (1998) Proc. Natl. Acad. Sci USA 95: 114-119 5. Yoshimura A (1998) Cytokine Growth Factor Rev 9: 197-204 6. Minamoto S et al (1997) Biochem Biophys Res Commun 237: 79-83 7. Cohney SJ et al (1999) Mol. Cell Biol. 19: 4980-88. 8. Davey HW et al (1999) Mol. Cwll Endocrinol 158: 111-16. |
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