| CatalogCode: | NB100-63732 |
| ProductName: | CD34 CLASS II Antibody |
| Product Description: | Mouse Monoclonal anti-CD34 CLASS II (QBEND/10), conjugated to PE |
| Clone: | QBEND/10 |
| Clonality: | Monoclonal |
| Immunogen: | Human endothelial cell membrane vesicles |
| Specificity: | NB100-63732 recognises the CD34 antigen - a single chain transmembrane glycoprotein of 105 - 120kD. Expressed on haemopoietic cells, including bone marrow and blood. It is expressed on the majority of cells in acute lymphoid leukaemia (ALL) as well as cells in acute myelogenous leukaemia (AML). It is also found on capillary endothelial cells. There are three classes of CD34 epitopes defined by differential sensitivity to enzymatic cleavage with glycoprotease from Pasteurella haemolytica and with neuraminidase.QBEND/10 specifically recognises a class II epitope. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 100 tests protein A purified Mouse ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Background: | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline, 1% Bovine Serum Albumin |
| ListPrice: | 295 |
| AppSummary: | FACS |
| SpeciesSummary: | Hu |
| ProteinTarget: | CD34 CLASS II (NOT FOR SALE IN USA) |
| PackageSize: | 100 tests |
| Conjugate: | PE |
| GeneralRef: | 1. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of Rhesus monkey. Cytometry 29: 351-362. 2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110 |
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