| CatalogCode: | NB100-63654 |
| ProductName: | CD28 Antibody |
| Product Description: | Mouse Monoclonal anti-CD28 (JJ319), conjugated to FITC |
| Clone: | JJ319 |
| Clonality: | Monoclonal |
| Specificity: | NB100-63654 recognizes the rat cell surface antigen CD28, a type I transmembrane glycoprotein, expressed by the majority of T lymphocytes and a subset of NK cells.CD28 is an essential costimulatory receptor for T cell activation and a modulator of T-cell cytokine production. Ligands of CD28 include CD80 (B7-1), CD86 (B7-2) and P13-kinase.Clone JJ319 has been identified as a highly effective co-stimulator of T cell proliferation and IL-2 secretion by resting T cells (1). |
| CrossReactivity: | Rat. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Localization: | Type I membrane protein. |
| Background: | Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline, pH7.4, 1% Bovine Serum Albumin |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Rt |
| ALTnames: | Anti-CD 28 antibody; Anti-CD28 antigen antibody; Anti-CD28 molecule antibody; Anti-MGC138290 antibody; Anti-T cell antigen CD28 antibody; Anti-T cell specific surface glycoprotein antibody; Anti-T cell specific surface glycoprotein CD28 antibody; Anti-Tp44 antibody |
| ProteinTarget: | CD28 |
| PackageSize: | 0.1 mg |
| Conjugate: | FITC |
| GeneralRef: | 1. Tacke, M. et al. (1995) Cellular distribution and costimulatory function of rat CD28. J. Immunol. 154: 5121-5127. 2. Tacke, M. et al. (1997) CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur. J. Immunol. 27: 239-247. |
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