| CatalogCode: | NB100-63637 |
| ProductName: | CD25 Antibody |
| Product Description: | Rat Monoclonal anti-CD25 (PC61.5.3), conjugated to PE |
| Clone: | PC61.5.3 |
| Clonality: | Monoclonal |
| Immunogen: | B6.1 CTL cell line. |
| Specificity: | NB100-63637 recognises mouse CD25 (IL-2 receptor alpha chain), expressed by activated T and B cells. |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Rat ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Background: | The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Rat |
| Buffer: | Phosphate buffered saline, 0.5% Bovine Serum Albumin |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Mu |
| ALTnames: | Anti-IL-2R ALPHA CHAIN antibody |
| ProteinTarget: | CD25 |
| PackageSize: | 0.5 ml |
| Conjugate: | PE |
| GeneralRef: | 1. Lowenthal, J.W. et al. (1985) . High and low affinity IL-2 receptors: analysis by IL-2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61. J. Immunol. 135: 3988-3994. 2. Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody. Eur. J. Immunol. 16: 317-320. 3. Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site. Eur. J. Immunol. 17: 929-935. |
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