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| Catalog Code: | NB100-63634 |
| Product Name: | CD25 Antibody |
| Product Description: | Mouse Monoclonal anti-CD25 (OX-39), conjugated to FITC |
| Clone: | OX-39 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.1 mg/ml |
| Immunogen: | Stimulated Rat T cells |
| Specificity: | NB100-63634 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1). |
| Cross Reactivity: | Rat. Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Background: | The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Phosphate buffered saline, pH7.4, 1% Bovine Serum Albumin |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Rt |
| Alternate Names: | Anti-IL-2R ALPHA CHAIN antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.09% Sodium Azide |
| Conjugate: | FITC |
| General References: | 1. Paterson, D.J. et al. (1987) Antigens of Activated Rat T Lymphocytes including a molecule of 50,000 Mr, detected only on CD4 positive T Blasts. Mol. Immunol. 24: 1281-1290. 2. Hayosh, N.S. and Swanborg, R.H. (1987) IX Inhibition of Adoptive Transfer of Autoimmune Encephalomyelitis with a monoclonal antibody specific for interleukin 2 Receptors. J. Immunol. 138: 3771-3775. 3. Tellides, G. et al. (1987) Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy. Transpl. Proc. XIX: 4231-4233. |
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