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| Catalog Code: | NB100-63367 |
| Product Name: | CD11c Antibody |
| Product Description: | Hamster Monoclonal anti-CD11c (N418), conjugated to FITC |
| Clone: | N418 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.1 mg/ml |
| Immunogen: | Mouse spleen dendritic cells. |
| Specificity: | NB100-63367 recognises the murine homologue of human CD11c, which is a 150/90kD member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (3). |
| Cross Reactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Hamster ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Localization: | Membrane; single pass type I membrane protein. |
| Background: | CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukaemia. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host Name: | Hamster |
| Buffer: | Phosphate buffered saline, pH7.4, 1% Bovine Serum Albumin |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Mu |
| Package Size: | 0.1 mg |
| Preservative: | 0.09% Sodium Azide |
| Conjugate: | FITC |
| General References: | 1. Crowley, M. T. et al. (1990) Use of fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J. Immunol. Methods. 133: 55 2. Metlay, J. P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753 3. Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step dendritic cell-targeted immunization. Proc. Natl. Acad. Sci. 97: 847 - 852. |
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