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| Catalog Code: | NB100-63329 |
| Product Name: | CD104 Antibody |
| Product Description: | Rat Monoclonal anti-CD104 (346-11A), conjugated to PE |
| Clone: | 346-11A |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | TSP-180 purified from Balb/c murine carcinoma. |
| Specificity: | NB100-63329 recognises mouse integrin beta 4 (CD104), a 200kD glycoprotein that can associate with integrin alpha 6 (CD49f) to form a heterodimer on the cell surface. Expression of CD104 is localised to the basal region of a wide variety of epithelial cells. In mice, CD104 is also expressed on peripheral neurones, carcinoma cell lines and a subset of endothelial cells. The alpha 6 beta 4 (CD49f/CD104) integrin has been identified as a component of hemidesmosomes in stratified squamous epithelium where it plays an important role in the adhesion of epithelia to basement membrane. Studies suggest that the CD49f/CD104 integrin is involved in cell matrix adhesion but expression of the CD49f/CD104 integrin in endothelial cells is thought to have a different function. |
| Cross Reactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 100 tests protein G purified Rat ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Localization: | Cell Membrane; single pass type I membrane protein |
| Background: | Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2a |
| Host Name: | Rat |
| Buffer: | Phosphate buffered saline, pH7.4, 1% Bovine Serum Albumin |
| Application Summary: | flow cytometry / FACS analysis |
| Species Summary: | Mu |
| Alternate Names: | Anti-CD104 antigen antibody; Anti-GP150 antibody; Anti-ITG B4 antibody; Anti-ITGB 4 antibody; Anti-ITGB4 antibody |
| Package Size: | 100 tests |
| Preservative: | 0.09% Sodium Azide |
| Conjugate: | PE |
| General References: | 1. Kennel, S.J. et al. (1992) The beta 4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice. J. Cell. Sci.101:145 - 150. 2. Kennel, S.J. et al. (1989) Analysis of the Tumor-associated Antigen TSP-180. Indentity with alpha 6 - beta 4 in the integrin superfamily. J. Biol. Chem. 264:15515 - 15521. |
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more information: | company product webpage |