| CatalogCode: | NB100-63324 |
| ProductName: | CD103 Antibody |
| Product Description: | Mouse Monoclonal anti-CD103 (LF61), conjugated to FITC |
| Clone: | LF61 |
| Clonality: | Monoclonal |
| Immunogen: | Hairy cell leukaemia cells |
| Specificity: | NB100-63324 recognises the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alphaE integrin. |
| CrossReactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | This antibody is useful for flow cytometry / FACS analysis |
| Localization: | Type I membrane protein. |
| Background: | CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline, 0.5% Bovine Serum Albumin |
| ListPrice: | 295 |
| AppSummary: | FACS |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-Antigen CD103 antibody; Anti-CD 103 antibody; Anti-CD103 antibody; Anti-CD103 antigen antibody; Anti-HML 1 antigen antibody; Anti-HML1 antigen antibody; Anti-Human mucosal lymphocyte antigen 1 antibody; Anti-Huminae antibody; Anti-Integrin alpha E heavy chain antibody; Anti-Integrin alpha E light chain antibody; Anti-Integrin alpha IEL antibody; Anti-ITGAE antibody; Anti-MGC141996 antibody; Anti-Mucosal lymphocyte 1 antigen antibody; Anti-Mucosal lymphocyte antigen 1 antibody |
| ProteinTarget: | CD103 |
| PackageSize: | 0.1 mg |
| Conjugate: | FITC |
| GeneralRef: | 1. Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kD, 125kD, 105kD) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459. 2. Falini, B. et al. (1990) Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients. Br. J. Haem. 76: 460-468. |
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