| CatalogCode: | NB100-63258 |
| ProductName: | IL-10 Antibody |
| Product Description: | Rat Monoclonal anti-IL-10 (JE-S5-16E-3) |
| Clone: | JE-S5-16E-3 |
| Clonality: | Monoclonal |
| Immunogen: | T cell derived murine IL-10 |
| Specificity: | NB100-63258 is specific for mouse Interleukin-10 (IL-10). This immunoregulatory cytokine is expressed by (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells. |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.5 mg Affinity purified Rat ascites. |
| Uses: | This antibody is useful for ELISA, Western Blot, flow cytometry / FACS analysis |
| Localization: | Secreted |
| Background: | Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 160 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG2b |
| Host_Name: | Rat |
| Buffer: | Phosphate buffered saline, pH7.4 |
| ListPrice: | 325 |
| AppSummary: | ELISA, WB, FACS |
| SpeciesSummary: | Mu |
| ALTnames: | Anti-CSIF antibody; Anti-Cytokine Synthesis Inhibitory Factor antibody; Anti-IL 10 antibody; Anti-IL10A antibody; Anti-Interleukin 10 antibody; Anti-Interleukin10 antibody; Anti-MGC126450 antibody; Anti-MGC126451 antibody; Anti-TGIF antibody |
| ProteinTarget: | IL-10 |
| PackageSize: | 0.5 mg |
| GeneralRef: | 1. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development. Immunol. Rev. 127: 5-24. 2. Litton M, et al. (1994) Early expression of cytokines in lymph nodes after treatment in vivo with Staphylcoccus enterotoxin B. J. Immunol. Methods. 175: 47-58. 3. Harizi, H. et al. (2002) Cyclooxygenase - 2 - issued prostaglandin E2 enhances the production of endogenous IL-10, which down-regulates dendritic cell functions. J. Immunol. 168:2255-2263. |
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