| CatalogCode: | NB100-63196 |
| ProductName: | MDM2 Antibody |
| Product Description: | Rabbit Polyclonal anti-MDM2 [Ser185] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide corresponding to aa 177-195 of mouse Mdm2. |
| Specificity: | NB100-63196 detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein. |
| CrossReactivity: | Mouse. Not yet tested in other species. |
| Packaging: | 0.05 mg Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for ELISA, Western Blot |
| Localization: | Nuclear and cytoplasmic. |
| Background: | MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53 mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2 p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Mu |
| ALTnames: | Anti-Double minute 2 protein antibody; Anti-Hdm 2 antibody; Anti-HDM2 antibody; Anti-MDM 2 antibody; Anti-Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody; Anti-MDM2BP antibody; Anti-Mouse Double Minute 2 antibody; Anti-MTBP antibody; Anti-Murine Double Minute Chromosome 2 antibody; Anti-Oncoprotein Mdm2 antibody; Anti-p53 Binding Protein Mdm2 antibody; Anti-Ubiquitin protein ligase E3 Mdm2 antibody |
| ProteinTarget: | MDM2 (pSer185) |
| PackageSize: | 0.05 mg |
| GeneralRef: | 1. Sdek, P. et al. (2004) The Central Acidic Domain of MDM2 Is Critical in Inhibition of Retinoblastoma-mediated Suppression of E2F and Cell Growth. J. Biol. Chem. 279: 53317 - 53322. 2. Feng J. et al. (2004) Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation. J. Biol. Chem. 279(34):35510-7. |
| Phosphorylated: | Ser185 |