| request information: |
|
| Catalog Code: | NB100-63114 |
| Product Name: | BMP-4 Antibody |
| Product Description: | Mouse Monoclonal anti-BMP-4 (3H2) |
| Clone: | 3H2 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Recombinant Human BMP-4 expressed in E.coli. |
| Specificity: | NB100-63114 recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 4 (BMP-4/BMP-2b), full-length 408aa. BMP-4 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis. BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation. Research studies have verified the particular importance of BMP-4 in epithelial-mesenchymal interactions, including its vital role in the early stages of tooth morphogenesis (3), the interaction between BMP-4 and its antagonist noggin in hair follicles (4) and the effects of BMP-4 expression/signaling in organs, including the lungs (5) and eyes (6) |
| Cross Reactivity: | Human. Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse ascites. |
| Uses: | This antibody is useful for ELISA, Western Blot |
| Localization: | Secreted, extracellular matrix. |
| Background: | Bone Morphogenetic Proteins (BMPs) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. Mature BMPs are 30-38 kDa proteins that assume a TGF beta like cysteine knot configuration. Unlike TGF beta, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported. Recently it was found that lovostatin, widely used for lowering cholesterol, also increases bone formation by turning on a gene (bmp2) that promotes local bone formation. BMPs create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor in the development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis. BMPs also appear to be responsible for normal dorsal/ventral patterning. BMPs are found in tissues that induce bone or cartilage growth, such as demineralized bone and urinary epithelium. Cellular responses to BMP5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Buffer: | Phosphate buffered saline, pH7.2 |
| Application Summary: | ELISA, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | Anti-BMP 2B antibody, Anti-BMP 4 antibody, Anti-BMP2B antibody, Anti-BMP2B1 antibody, Anti-Bone morphogenetic protein 4 antibody, Anti-DVR4 antibody, Anti-ZYME antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.09% Sodium Azide |
| General References: | 1. Balemans, W. and Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators. Dev. Biol. 250: 231-250. 2. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction. Biol. Chem. 385: 697-710. 3. Feng, J.Q. et al. (2002) Identification of Cis-DNA regions controlling Bmp4 expression during tooth morphogenesis in vivo. J. Dent. Res. 81(1): 6-10. 4. Botchkarev, V.A. et al. (2001) Noggin is required for induction of the hair follicle growth phase in postnatal skin. Faseb J. 15: 2205-2214. 5. Bellusci, S. et al. (1996) Evidence from normal expression and targeted misexpression that Bone Morphogenetic Protein (Bmp-4) plays a role in mouse embryonic lung morphogenesis. Developmental 122: 1693-1702. 6. Trousse, F. et al. (2001) BMP4 mediates apoptotic cell death in the developing chick eye. J. Neuroscience 21: 1292-1301. |
order or
more information: | company product webpage |
|