| CatalogCode: | NB100-60942 |
| ProductName: | SUPT16H Antibody |
| Product Description: | Goat Polyclonal anti-FACT / SUPT16H |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, C-DLTNKEGKKPEEK, representing the internal region of the sequence according to NP_009123.1 |
| Epitope: | DLTNKEGKKPEEK |
| CrossReactivity: | Human. Other species not tested. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Control: | HeLa cell lysate |
| Background: | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline [pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-SUPT16H antibody; anti-suppressor of Ty 16 homolog (S. cerevisiae) antibody; anti-HGNC:11465 antibody; anti-CDC68 antibody; anti-FACT antibody; anti-FACTP140 antibody; anti-FLJ10857 antibody; anti-FLJ14010 antibody; anti-SPT16/CDC68 antibody; anti-chromatin-specific transcription elongation factor large subunit antibody |
| ProteinTarget: | SUPT16H |
| PackageSize: | 0.1 mg |
| Homology: | Human |
| GeneralRef: | Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D. FACT facilitates transcription-dependent nucleosome alteration. Science. 2003 Aug 22;301(5636):1090-3. PMID: 12934006 |
| Gene_Id: | 11198 |
| Gene_Symbol: | SUPT16H |
| Ref_Sqnce: | NP_009123.1 |
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