| CatalogCode: | NB100-59750 |
| ProductName: | ACACB Antibody |
| Product Description: | Goat Polyclonal anti-ACACB |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, C-QLGEPDLSDKDRKD, representing the internal region of the sequence according to NP_001084.2 |
| Epitope: | QLGEPDLSDKDRKD |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:32,000.Western Blot: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277kDa according to NP_001084.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Background: | *Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline [pH7.3] with 0.5% BSA |
| ListPrice: | 195 |
| AppSummary: | ELISA |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ACACB antibody, anti-acetyl-Coenzyme A carboxylase beta antibody, anti-ACCB antibody, anti-HACC275 antibody, anti-acetyl-CoA carboxylase 2 antibody |
| ProteinTarget: | ACACB |
| PackageSize: | 0.1 mg |
| Homology: | Human |
| GeneralRef: | Reznick RM, Zong H, Li J, Morino K, Moore IK, Yu HJ, Liu ZX, Dong J, Mustard KJ, Hawley SA, Befroy D, Pypaert M, Hardie DG, Young LH, Shulman GI. Aging-Associated Reductions in AMP-Activated Protein Kinase Activity and Mitochondrial Biogenesis. Cell Metab. 2007 Feb;5(2):151-6. PMID: 17276357 |
| Gene_Id: | 32 |
| Gene_Symbol: | ACACB |
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