| CatalogCode: | NB100-57845 |
| ProductName: | OGT Antibody |
| Product Description: | Goat Polyclonal anti-OGT |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, C-YEHPKDLKLSDGR, representing the internal region of the human sequence according to NP_858058.1; NP_858059.1 |
| Epitope: | YEHPKDLKLSDGR |
| CrossReactivity: | Rat and mouse. Other species not tested. Expected based upon homology: Human, Dog |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:64,000.Western Blot: Approx 110kDa band observed in Pancreas lysates from Rat and Mouse (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2ug/ml.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Control: | Rat pancreas lysate |
| Background: | O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline [ pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Rt, Mu |
| ALTnames: | anti-OGT antibody, anti-O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, anti-FLJ23071 antibody, anti-HRNT1 antibody, anti-MGC22921 antibody, anti-O-GLCNAC antibody, anti-O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase antibody, anti-uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody |
| ProteinTarget: | OGT |
| PackageSize: | 0.1 mg |
| Homology: | Human, Mouse, Rat, Dog |
| GeneralRef: | Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS. Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.Diabetes. 2004 Dec;53(12):3184-92. PMID: 15561949 |
| NotesMain: | This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1 Other Gene ID: 108155 (mouse); 26295 (rat); |
| Gene_Id: | 8473 |
| Gene_Symbol: | OGT |