| CatalogCode: | NB100-56599 |
| ProductName: | PARP Antibody |
| Product Description: | Mouse Monoclonal anti-PARP (194C1439) |
| Clone: | 194C1439 |
| Clonality: | Monoclonal |
| Immunogen: | This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP. |
| Specificity: | This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain. |
| CrossReactivity: | Human Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | WB: 1-2 ug/ml~; FC (Intracellular): 1-3 ug/test |
| Control: | camptothecin treated HL-60 (12 hr) |
| Background: | The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | PARP |
| PackageSize: | 0.1 mg |