| CatalogCode: | NB100-56597 |
| ProductName: | IkBa Antibody |
| Product Description: | Mouse Monoclonal anti-IkBa (5D1623) |
| Clone: | 5D1623 |
| Clonality: | Monoclonal |
| Immunogen: | This monoclonal antibody was developed against a synthetic peptide corresponding to the caspase-3 mediated cleavage site of IkBa. |
| CrossReactivity: | Human Not yet tested in other species. |
| Packaging: | 0.1 mg of Mouse Affinity Purified |
| Uses: | WB: 1 ug/ml |
| Control: | anti-Fas treated Jurkat (30 min) |
| Background: | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity Purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | IkBa |
| PackageSize: | 0.1 mg |