| CatalogCode: | NB100-56565 |
| ProductName: | Caspase-1 Antibody |
| Product Description: | Mouse Monoclonal anti-Caspase-1 (14F468) |
| Clone: | 14F468 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide corresponding to amino acids 371-390 [BLAST]RKVRFSFEQPDGRAQMPTTE[/BLAST] of human caspase-1 was used as immunogen. It will cross-react with mouse and rat Caspase-1, based on NCBI BLAST. |
| Specificity: | In HeLa, a 45 kDa band is observed. In The antibody recognizes full-length Caspase-1. The antibody also recognizes cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein. |
| CrossReactivity: | Human, Mouse Not yet tested in other species. |
| Packaging: | 0.1 mg of Mouse Affinity Purified |
| Uses: | WB: 0.5-2 ug/ml~; IHC (paraffin): researchers should titrate to determine appropriate concentration for their model system.~; |
| Control: | HeLa or NIH 3T3 |
| Background: | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity Purified |
| Isotype: | IgG1, k |
| Host_Name: | Mouse |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Mu |
| ProteinTarget: | Caspase-1 |
| PackageSize: | 0.1 mg |
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