| CatalogCode: | NB100-56564 |
| ProductName: | Caspase-1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase-1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide corresponding to amino acids 31-45 [BLAST]QTRVLNKEEMEKVKR[/BLAST] of human Caspase-1 was used as immunogen. The antibody will cross-react with mouse Caspase-1, based on NCBI BLAST. |
| Specificity: | In HeLa, a 45 kDa band is observed. The antibody recognizes full-length Caspase-1. The antibody also recognizes cleaved caspase-1 forms that include amino acids 31-45 of the Caspase-1 protein. |
| CrossReactivity: | Human Not yet tested in other species. |
| Packaging: | 0.1 mg of Rabbit Affinity Purified |
| Uses: | WB: 0.5-2 ug/ml~; IHC (paraffin): researchers should titrate to determine appropriate concentration for their model system. |
| Background: | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity Purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | Caspase-1 |
| PackageSize: | 0.1 mg |
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