| CatalogCode: | NB100-56465 |
| ProductName: | Caspase-3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase-3 |
| Clonality: | Polyclonal |
| Immunogen: | A portion of amino acids 150-200 of human Caspase-3 was used as the immunogen. |
| Specificity: | The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, bovine, dog, monkey, sheep, pig and cat. |
| CrossReactivity: | Bovine, Cat, Chimpanzee, Chinese Hamster, Dog, Human, Macaque, Monkey Not yet tested in other species. |
| Packaging: | 0.1 mg of Rabbit Affinity Purified |
| Uses: | WB: 1-3 ug/ml |
| Background: | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. (5). Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa (3). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Bo, Fe, Mk, Ha, Ca, Hu |
| ProteinTarget: | Caspase-3 |
| PackageSize: | 0.1 mg |
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