| Catalog Code: | NB100-564 |
| Product Type: | Primary Antibody |
| Product Name: | MAD-2 Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 4085 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-MAD-2 |
| Cross Reactivity: | Human. Other species not tested. Reactivity with mouse MAD2 is unknown, but this ortholog shares all but 1 residue with human MAD2 at the site to which the epitope maps. |
| Species Summary: | Hu |
| Background: | The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segre |
| Alternate Names: | anti-MAD2 antibody, anti-HsMAD2 antibody, anti-Mitotic Arrest Deficient 2 antibody, anti-MAD2L1 antibody, anti-MAD2-like 1 antibody |
| Immunogen: | A synthetic peptide which maps to a region between residues 150 and the C-terminus (residue 205) of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085). |
| Specificity: | NB 100-564 is specific for human MAD2 protein |
| Packaging: | 0.1 ml of affinity purified rabbit sera in a Tris-citrate/phosphate (pH 7-8) buffer with 0.1% sodium azide. |
| Package Size: | 0.1 ml |
| Concentration: | 1 mg/ml |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:20,000 Immunoprecipitation: 2 to 4 ug/mg lysate |
| Application Summary: | WB, IP |
| Notes Main: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |