| CatalogCode: | NB100-56148 |
| ProductName: | NALP1 Antibody |
| Product Description: | Rabbit Polyclonal anti-NALP1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide corresponding to amino acids 161-180 (PSSPDHESPSQESPNAPTST) of human NALP1/NAC/CARD7 was used as immunogen, GenBank no. NP_127497.1. |
| CrossReactivity: | Human Not yet tested in other species. |
| Packaging: | 0.05 ml of Rabbit Whole antisera |
| Uses: | WB: 1:1000-1:2000~; IHC (paraffin): 1:1000-1:5000~; IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)~; IP: 1:50-1:200 |
| Control: | lymphoid, many cancer cell lines |
| Background: | NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. IMG-5735 recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at ~166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS containing 0.02% sodium azide. |
| ListPrice: | 295 |
| AppSummary: | IHC, IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-NAC antibody, anti-CARD7 antibody |
| ProteinTarget: | NALP1 |
| PackageSize: | 0.05 ml |
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