| CatalogCode: | NB100-56118 |
| ProductName: | Active/Cleaved Caspase-9 Antibody |
| Product Description: | Rabbit Polyclonal anti-Active/Cleaved Caspase-9 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant catalytically active human Caspase-9 protein was used as immunogen. |
| Specificity: | 1. Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. 2. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation. |
| CrossReactivity: | Dog, Gerbil, Human, Mouse, Rat Not yet tested in other species. |
| Packaging: | 0.05 ml Whole Rabbit antisera. |
| Control: | Cells or tissues undergoing apoptosis, lymphatic tissues, ischemic brain. |
| Background: | Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-9 polyclonal antisera preferntially recognizes active/cleaved caspase-9. Whereas the antisera has a strong preference for active/cleaved caspase-9, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-9. On western blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 295 |
| AppSummary: | IP, WB, IHC-P, IHC-Fr |
| SpeciesSummary: | Ca, Hu, Mu, Rt |
| ProteinTarget: | Active/Cleaved Caspase-9 |
| PackageSize: | 0.05 ml |
| GeneralRef: | 1. Siegel RM. 2006. Caspases at the crossroads of immune-cell life and death. Nature 6:308-317. 2. Lavrik IN, A Golks, and PH Krammer. 2005. Caspases: pharmacological manipulation of cell death. J Clin. Invest. 115:2665-2672. |
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