| CatalogCode: | NB100-56115 |
| ProductName: | Active/Cleaved Caspase-7 Antibody |
| Product Description: | Rabbit Polyclonal anti-Active/Cleaved Caspase-7 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant catalytically active human Caspase-7 protein was used as immunogen. |
| Specificity: | 1. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots ia considered to be a marker of caspase activation. IMG-5702 preferentially detects active Caspase-7 (large subunit: 17-22 kDa, and small subunit: 10 kDa). However, it may also detect pro-caspase-7 (~35 kDa) in some cell or tissue systems. |
| CrossReactivity: | Gerbil, Human, Mouse, Rat Not yet tested in other species. |
| Packaging: | 0.05 ml of Rabbit Whole antisera |
| Uses: | IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents) |
| Control: | Cells or tissues undergoing apoptosis, cancer cell lines. |
| Background: | Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-7 polyclonal antisera recognizes the large (~17-22 kDa) and small (~10 kDa) subunits of active/cleaved caspase-7. Whereas the antisera has a strong preference for active/cleaved caspase-7, in some cell or tissue systems or techniques the antisera may also recognize the proform of Caspase-7 (~35 kDa). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 295 |
| AppSummary: | IP, WB, IHC-P, IHC-Fr |
| SpeciesSummary: | Hu, Mu, Rt |
| ProteinTarget: | Active/Cleaved Caspase-7 |
| PackageSize: | 0.05 ml |
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