| CatalogCode: | | NB100-56074 |
| ProductName: | | Apaf 1 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Apaf 1 |
| Clonality: | | Polyclonal |
| Immunogen: | | Full-length recombinant human Apaf1 protein was used as immunogen. |
| CrossReactivity: | | Gerbil, Human, Mouse, Rat Not yet tested in other species. |
| Packaging: | | 0.05 ml of Rabbit Whole antisera |
| Uses: | | WB: 1:1000-1:2000~; IHC (paraffin): 1:1000-1:5000~; IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)~; IP: 1:50-1:200 |
| Control: | | spleen, lymphoid, many cancer cell lines |
| Background: | | Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is a ~130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001). |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Whole antisera |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS containing 0.02% sodium azide. |
| ListPrice: | | 295 |
| AppSummary: | | IHC, IP, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ProteinTarget: | | Apaf 1 |
| PackageSize: | | 0.05 ml |
| more information: | | company product webpage |
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