| CatalogCode: | NB100-56065 |
| ProductName: | IkBa Antibody |
| Product Description: | Mouse Monoclonal anti-IkBa (39A1413) [Ser32/Ser36], conjugated to Biotin |
| Clone: | 39A1413 |
| Clonality: | Monoclonal |
| Immunogen: | This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa. |
| Specificity: | For detection of non-phosphroylated form of IkBa, Cat. # IMG-127 should be used. |
| CrossReactivity: | Human, Mouse Not yet tested in other species. |
| Packaging: | 0.1 mg Mouse ascites. |
| Uses: | WB: 1 ug/ml ~; IP: 1 ug/ml~; ELISA: 1:100-1:1000 |
| Control: | TNFa treated Jurkat |
| Background: | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG1, k |
| Host_Name: | Mouse |
| Buffer: | PBS containing 0.05% sodium azide. Sodium azide is highly toxic |
| ListPrice: | 295 |
| AppSummary: | ELISA |
| SpeciesSummary: | Hu, Mu |
| ProteinTarget: | IkBa |
| PackageSize: | 0.1 mg |
| Conjugate: | Biotin |
| Phosphorylated: | Ser32/Ser36 |
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