| CatalogCode: | NB100-55784 |
| ProductName: | IKKe Antibody |
| Product Description: | Mouse Monoclonal anti-IKKe (72B587), conjugated Biotin |
| Clone: | 72B587 |
| Clonality: | Monoclonal |
| Immunogen: | This antibody was raised against a synthetic peptide corresponding to amino acids 175-188 of human IKKi/e protein. It will cross-react with mouse and rat IKKi/e and TBK1. |
| Specificity: | In WB, a 80 kDa band is observed. |
| CrossReactivity: | Human, Mouse, Rat Not yet tested in other species. |
| Packaging: | 0.1 mg of Mouse sera |
| Uses: | WB: 1-3 ug/ml |
| Control: | Daudi, HeLa, or NIH 3T3 |
| Background: | NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG1, k |
| Host_Name: | Mouse |
| Buffer: | PBS containing 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-IKKi antibody |
| ProteinTarget: | IKKe |
| PackageSize: | 0.1 mg |
| Conjugate: | Biotin |
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