| CatalogCode: | NB100-546 |
| ProductName: | PLK1 Antibody |
| Product Description: | Rabbit Polyclonal anti-PLK1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide which maps to a region between residues 1 and 50 of human Polo-like kinase 1 using the numbering given in SwissProt entry P53350 (GeneID 5347). |
| Specificity: | NB 100-546 is specific for human PLK1 protein. |
| CrossReactivity: | Human. Other species have not been tested. Reactivity with mouse and Xenopus PLK1 is likely owing to conservation of sequence between these orthologs and human PLK1 at the site to which the epitope maps. |
| Packaging: | 0.1 ml Rabbit antisera. |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:500 - 1:2,500 Immunoprecipitation: 2 to 4 ug/mg lysate. IP with NB 100-546 is less efficient than NB 100-547 and NB 100-548. Histo/Cytochemistry: not tested*In some cases, the antibody may be diluted further than indicated. |
| Background: | Polo-like kinases are a family of serine/threonine protein kinases, named after the prototypic founding member of the family, the polo gene product of Drosophila melanogaster. The polo kinase was originally identified in mutants that display abnormal mitotic spindle organization. Subsequently, potential homologues of Drosophila polo have been identified in yeasts (Cdc5p in Saccharomyces cerevisiae; plo1+ in Schizosaccharmoyces pombe) and in mammals (polo-like kinase 1; Plk1). Genetic and biochemical studies suggest that polo, Cdc5p and plo1+ may be required for mitotic spindle organization and, possibly, for cytokinesis. Likewise, the patterns of expression, activity and subcellular localization of Plk1 suggest that Plk1 may function during mitosis in spindle assembly and function. Recent studies have demonstrated that mammalian Plks regulate the function of the Golgi complex, a cellular organelle closely associated with the centrosome and also have microtubule organization activity. Furthermore, deregulated expression of human PLK1 and PLK3 is strongly correlated with the development of many types of malignancies, and ectopic expression of kinase-active Plk3 or Plk1 dominant negative protein leads to rapid cell death. |
| Storage: | Store at 4C. Do not freeze. |
| Host_Name: | Rabbit |
| ListPrice: | 285 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PLK1 antibody, anti-PLK-1 antibody, anti-Polo-like kinase 1 antibody, anti-STPK13 antibody, anti-Serine-threonine kinase 13 antibody |
| ProteinTarget: | PLK-1 antibody |
| PackageSize: | 0.1 ml |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |
| Gene_Id: | 5347 |
| Omim_ID: | NB100-548 |