| CatalogCode: | NB100-543 |
| ProductName: | RPA32 Antibody |
| Product Description: | Rabbit Polyclonal anti-RPA32 [Ser4/Ser8] |
| Clonality: | Polyclonal |
| Immunogen: | A dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (LocusLink ID 6118). |
| Specificity: | NB 100-543 is specific for human RPA-32 pSer4/Ser8 protein. |
| CrossReactivity: | Human. Other species have not been tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 - 1:10,000 Immunoprecipitation: 4 to 8 ug/mg of lysate Immunofluorescence: 1 to 4 ug/ml*In some cases, the antibody may be diluted further than indicated. |
| Background: | Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA is a target for DNA dependent protein kinase (DNA-PK), Ataxia Telangiectasia Mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA 32, is phosphorylated during the S-phase of the cell cycle in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| ListPrice: | 330 |
| AppSummary: | WB, IP, IF |
| SpeciesSummary: | Hu |
| ALTnames: | anti-RPA2 antibody, anti-RPA32 antibody, anti-REPA2 antibody, anti-Replication protein A2 antibody, anti-32 kDA antibody, anti-RFA2 antibody, anti-RPA-p34 antibody |
| ProteinTarget: | RPA32 pSer4/Ser8 antibody |
| PackageSize: | 0.1 ml |
| Phosphorylated: | Ser4/Ser8 |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |