| CatalogCode: | NB100-53786 |
| ProductName: | CARD15 Antibody |
| Product Description: | Goat Polyclonal anti-CARD15 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, KFRFTDRERHCSPTD, representing the internal region of the human protein according to NP_071445.1 |
| CrossReactivity: | Human. Other species not tested. |
| Packaging: | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3ug/ml.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Control: | Human Peripheral Blood Mononucleocyte |
| Background: | CARD15 (Nod2) is a member of the Nod1/Apaf-1 family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and a C-terminal regulatory domain. CARD15 induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It also confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Unlike Nod1, this homologue is primary expressed in monocytes. Defects in CARD15 are associated with Blau Syndrome (BS) and Crohn's Disease (CD), and are a cause of susceptibility to ulcerative colitis. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Goat |
| Buffer: | Tris-saline [ pH7.3] with 0.5% BSA |
| ListPrice: | 295 |
| NovusRef: | Wang, S., et al. Microarray analysis of cytokine activation of apoptosis pathways in the thyroid. Endocrinology. 148: 4844-4852, 2007. |
| AppSummary: | ELISA, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CARD15 antibody, anti-caspase recruitment domain family antibody, anti-member 15 antibody, anti-ACUG antibody, anti-BLAU antibody, anti-CD antibody, anti-IBD1 antibody, anti-NOD2 antibody, anti-NOD2B antibody, anti-PSORAS1 antibody, anti-LRR-containing protein antibody, anti-NOD2 protein antibody, anti-caspase recruitment domain protein 15 antibody, anti-inflammatory bowel disease protein 1 antibody, anti-nucleotide-binding oligomerization domain 2 antibody |
| ProteinTarget: | CARD15 / NOD2 (Internal) |
| PackageSize: | 0.1 mg |
| Homology: | Human, Mouse, Rat |
| GeneralRef: | Keshav S.Paneth cells: leukocyte-like mediators of innate immunity in the intestine.J Leukoc Biol. 2006 Jun 22;PMID: 16793911 |
| Gene_Id: | 64127 |
| Gene_Symbol: | CARD15 |