| Catalog Code: | NB100-478 |
| Product Type: | Primary Antibody |
| Product Name: | PCNA Antibody |
| List Price: | 285 |
| Clonality: | Monoclonal |
| Gene ID: | 5111 |
| Host: | Mouse |
| Clone: | 339 |
| Isotype: | IgG |
| Product Description: | Mouse Monoclonal anti-PCNA (339) |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Alternate Names: | anti-Cyclin antibody, anti-DNA polymerase delta auxiliary protein antibody, anti-HGCN8729 antibody, anti-MGC8367 antibody, anti-polymerase delta accessory protein antibody, anti-Proliferating Cell Nuclear Antigen antibody |
| Immunogen: | Recombinant PCNA interdomain connector loop protein. This immunogen is comprised of residues 107-196 of human PCNA. (The C-terminal portion of this antigen does not extend beyond the true IDCL of PCNA, and as such, also includes part of the PCNA D2E2 lo |
| Localization: | Nuclear |
| Specificity: | This antibody reacts with the interdomain connector loop of human PCNA, other not determined. |
| Buffer: | PBS [pH 7.2] |
| Packaging: | 0.1 mg of Protein G purified mouse ascites in PBS, pH 7.2, no preservative added. |
| Package Size: | 0.1 mg |
| Concentration: | 1 mg/ml |
| Uses: | ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/500 - 1/1000. IF: See Malkas, et al. IHC-P: See Malkas, et al. Predicted molecular weight: 31.32 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determ |
| Application Summary: | ELISA, IHC, IF, WB |
| General References: | 1. Levin DS et al. Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base-excision repair. Curr Biol. 10:919-22 (2000). <PUBMED:10959839> 2. Warbrick E PCNA binding through a conserved motif. Bioes |
| Product References: | 1. Malkas, L. H., Herbert, B. S., Abdel-Aziz, W., Dobrolecki, L. E., Liu, Y., Agarwal, B., Hoelz, D., Badve, S., Schnaper, L., Arnold, R. J., Mechref, Y., Novotny, M. V., Loehrer, P., Goulet, R. J., and Hickey, R. J. (2006) A cancer-associated PCNA expres |
| Novus References: | 1. Malkas, L.H. et al. A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. PNAS 2006; 103; 19472-19477. |
|