| CatalogCode: | | NB100-457 |
| ProductName: | | PCNA Antibody |
| Product Description: | | Rabbit Polyclonal anti-PCNA |
| Clonality: | | Polyclonal |
| Immunogen: | | The epitope recognized by NB 100-457 maps to a region between residues 225-261 (C-terminus) of human proliferating cell nuclear antigen using the SwissProt entry P12004 (GeneID 5111). |
| Specificity: | | This antibody is specific for human PCNA. |
| CrossReactivity: | | NB 100-457 reacts with human protein. Other species have not been tested. Reactivity with mouse PCNA is likely owing to conservation between the mouse sequence and the peptide used for this antibody production. |
| Packaging: | | 0.1 ml Rabbit antisera. |
| Uses: | | NB 100-457 may be used for Western Blot and immunoprecipitation.Suggested working dilutions: *Western Blot - 1:5,000-1:20,000 (ECL)Immunocytochemistry - Not DeterminedImmunoprecipitation - 2-5 ug/mg lysate*The investigator should determine the optimal working dilution for a specific application. |
| Background: | | PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor for DNA polymerase delta. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This particular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur. |
| Storage: | | Store at 4C. Do not freeze. |
| Host_Name: | | Rabbit |
| Buffer: | | 0.2% Gelatin, PBS |
| ListPrice: | | 285 |
| AppSummary: | | WB, IP |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-Cyclin antibody, anti-DNA polymerase delta auxiliary protein antibody, anti-HGCN8729 antibody, anti-MGC8367 antibody, anti-polymerase delta accessory protein antibody, anti-Proliferating Cell Nuclear Antigen antibody |
| ProteinTarget: | | PCNA antibody |
| PackageSize: | | 0.1 ml |
| NotesMain: | | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |
| Gene_Id: | | 5111 |
| Omim_ID: | | NB100-456 |
| more information: | | company product webpage |