| CatalogCode: | NB100-439 |
| ProductName: | Separase Antibody |
| Product Description: | Mouse Monoclonal anti-Separase (XJ11-1B12) |
| Clone: | XJ11-1B12 |
| Clonality: | Monoclonal |
| Immunogen: | Maltose-Binding Protein fusion of a C-terminal fragment of human separase (residues 1866-1996). |
| CrossReactivity: | NB 100-439 recognizes human separase protein. Other species have not been tested. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Western Blot, where a band is seen at ~220 kDa (full-length form) and ~65 kDa (auto-processed form). |
| Localization: | Nuclear and Centrosomal |
| Background: | Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting down-regulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Caspase like protein ESPL1 antibody, anti-ESP1 antibody, anti-ESPL1 antibody, anti-Extra spindle poles like 1 antibody, anti-KIAA0165 antibody, anti-Separin antibody, anti-Similar to fission yeast cut1and gene antibody, anti-SSE antibody |
| ProteinTarget: | Separase antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Chestukhin, A., et al. Western blot screening for monoclonalantibodies against human separase. J. Immunol. Methods. 274:105-113, 2003. [Western blot, Human] 2. Chestukhin, A., et al. Processing,localization, and requirement of human separase for normal anaphase progression. PNAS. 100: 4574-4579, 2003. [Western blot, Immunofluorescence, Human] 3. Ross, K.E., & Cohen-Fix, O. Separase: a conserved protease separating more than just sisters. TRENDS in Cell Biology. 12(1), 2002. 4. Uhlmann, F. Separase regulation during mitosis. Biochem Soc. Symp. 70:243-251, 2003. 5. Uhlmann, F. Chormosome cohesion and separation: from men and molecules. Curr. Biol. 13:R104-R114, 2003. 6. Pellman, D., & Christman, M.F. Separase anxiety:dissolving the sister bond and more. Nature Cell Biol. 3: E207-E209, 2001. |
| Gene_Id: | 9700 |
| Omim_ID: | NB100-439SS |
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