| CatalogCode: | | NB100-40794 |
| ProductName: | | Chat Antibody |
| Product Description: | | Goat Polyclonal anti-Chat |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide, C-KERARGPTKPSNLD, representing the C Terminus of the protein according to NP_034021.1 |
| CrossReactivity: | | Mouse, Rat. |
| Packaging: | | 0.1 mg Immunogen affinity purified Goat antisera. |
| Uses: | | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 75-80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5ug/ml. |
| Control: | | Mouse Brain lysate |
| Background: | | Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfunction of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer's disease, Down and Rett syndromes. Choline acetyltransferase recruits choline for the synthesis of acetylcholine at preganglionic parasympathetic and sympathetic fibres. It is also present at parasympathetic postganglionic fibres. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Goat |
| Buffer: | | Tris-saline [ pH7.3] with 0.5% BSA |
| ListPrice: | | 295 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Mu, Rt |
| ALTnames: | | anti-Chat antibody, anti-choline acetyltransferase antibody, anti-B230380D24Rik antibody |
| ProteinTarget: | | Chat |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15. , PMID: 16539660 [PubMed - in process] |
| NotesMain: | | Gene ID = (human); 12647 (mouse); 432359 (rat); This antibody is NOT expected to cross-react to Human CHAT. |
| Gene_Id: | | 12647/432359 |
| more information: | | company product webpage |