| CatalogCode: | NB100-406SS |
| ProductName: | 14-3-3 gamma Antibody |
| Product Description: | Mouse Monoclonal anti-14-3-3 gamma (KC21) |
| Clone: | KC21 |
| Clonality: | Monoclonal |
| Immunogen: | This antibody was raised against an N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated. |
| Specificity: | This antibody is specific for human 14-3-3 gamma. |
| CrossReactivity: | NB 100-406 reacts with the naturally occurring human form of 14-3-3 gamma, where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody. Other species have not been tested. |
| Packaging: | 0.025 ml Whole antisera Mouse ascites. |
| Uses: | This antibody can be used in Western Blot where a distinct band around 33 kDa is detected.Suggested working dilutions: *Western Blot - 1:3,000*The investigator should determine the optimal working dilution for a specific application. |
| Background: | 14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a protein's function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| ListPrice: | 100 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Protein kinase C inhibitor protein 1 antibody, anti-KCIP-1 antibody |
| PackageSize: | 0.025 ml |
| GeneralRef: | 1. Ferl, R.J. 14-3-3 proteins:regulation of signal-induced events. Physiol. Plant. 120(2):173-178, 2004. 2. Hermeking, H. The 14-3-3 cancer connection. Nature. 3:931-943, 2003. 3. Jin, Z., et al. Nicotine induces multi-site phosphorylation of Bad in association with suppression of apoptosis. J. Biol. Chem. 279(22):23837-23844, 2004. (Immunoprecipitation, western blot, human) |