| CatalogCode: | | NB100-39002 |
| ProductName: | | FOXP3 Antibody |
| Product Description: | | Rabbit Polyclonal anti-FOXP3 |
| Clonality: | | Polyclonal |
| Immunogen: | | A fusion protein, GST-mouse Foxp3-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein. |
| CrossReactivity: | | This antibody has shown reactivity against mouse protein (CD4+CD25+ T cells). There appears to be no reactivity against human Jurkat and mouse CD4+CD25- T cell lysates. Other species and samples have not been tested. |
| Packaging: | | 0.1 ml protein G purified Rabbit antisera. |
| Uses: | | This antibody can be used for Western blot analysis, where a band is seen ~ 48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. This antibody will also immunopreciptate transfected FOXP3-HA tagged proteins. |
| Localization: | | Nuclear |
| Control: | | Mouse CD4+CD25+ T cells |
| Background: | | FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | protein G purified |
| Host_Name: | | Rabbit |
| Buffer: | | PBS + 50% glycerol |
| ListPrice: | | 285 |
| AppSummary: | | FACS, WB |
| SpeciesSummary: | | Mu |
| ALTnames: | | anti-AIID antibody; anti-DIETER antibody; anti-Forkhead box P3 antibody; anti-foxp3 antibody; anti-IPEX antibody; anti-JM2 antibody; anti-PIDX antibody; anti-SCURFIN antibody; anti-XPID antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Lim, H.W., et al. Cutting edge: direct suppression of B cells by CD4+CD25+ regulatory T cells. J. Immunol. 175:4180-4183 (2005) 2. Polanczyk MJ, et al. Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol. 173(4:2227-30 (2004) 3. Ochs, HD, et al. FOXP3 acts as a rheostat of the immune response. Immunol Rev. 203:156-64 (2005) |
| more information: | | company product webpage |