| Catalog Code: | NB100-383 |
| Product Type: | Primary Antibody |
| Product Name: | H2AX Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 3014 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-H2AX |
| Cross Reactivity: | NB 100-383 recognizes human and mouse H2AX. |
| Species Summary: | Hu, Mu |
| Background: | Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of m |
| Alternate Names: | anti-Histone H2AX antibody, anti-Histone H2AFX antibody, anti-Histone H2A.X antibody, anti-Histone H2A/X antibody |
| Immunogen: | A synthetic peptide made to a portion of the C-terminus of human histone H2AX. |
| Specificity: | This antibody is specific for H2AX. It is not known if this antibody also recognizes the phosphorylated form of H2AX. |
| Purity: | IgG purified |
| Buffer: | Tris-citrate/phosphate [pH7-8] |
| Packaging: | 0.1 ml of rabbit antisera (1 mg/ml) |
| Package Size: | 0.1 ml |
| Concentration: | 1 mg/ml |
| Uses: | By Western blot, this antibody recognizes a band at ~25 kDa, representing monoubiquinated H2AX protein and ~14 kDa, representing H2AX. |
| Application Summary: | IP, WB |
| Product References: | 1. Hovest, M. G., Bruggenolte, N., Hosseini, K. S., Krieg, T., and Herrmann, G. (2006) Senescence of Human Fibroblasts after Psoralen Photoactivation Is Mediated by ATR Kinase and Persistent DNA Damage Foci at Telomeres, 17, 1758-1767. |
| Novus References: | 1. Masutomi, K., et al. The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. PNAS. 102(23): 8222-8227, 2005. (Western blot, human) |
| Notes Main: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |