| CatalogCode: | | NB100-347 |
| ProductName: | | RPA32 Antibody |
| Product Description: | | Rabbit Polyclonal anti-RPA32 |
| Clonality: | | Polyclonal |
| Immunogen: | | Recombinant protein encoding full-length human RPA-32 expressed in E. coli. |
| CrossReactivity: | | NB 100-347 reacts with human and mouse. Other species have not been tested. |
| Packaging: | | 0.1 ml Whole Rabbit antisera. |
| Uses: | | NB 100-347 may be used for Western Blot. Other applications have not been tested.Suggested working dilutions: *Western Blot - 1:1,000 (ECL)ELISA - NDImmunoprecipitation - ND*The investigator should determine the optimal working dilution for a specific application. |
| Control: | | HeLa, Raji, 3T3 whole cell lysates and recombinant fusion protein |
| Background: | | Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutaed gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during S-phase of the cell cycle, in response to DNA damage and during apoptosis of Jurkat T-lymphocytes. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Whole antisera |
| Host_Name: | | Rabbit |
| Buffer: | | N/A |
| ListPrice: | | 295 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu, Mu |
| ALTnames: | | anti-RPA2 antibody, anti-RPA32 antibody, anti-REPA2 antibody, anti-Replication protein A2 antibody, anti-32 kDA antibody, anti-RFA2 antibody, anti-RPA-p34 antibody |
| ProteinTarget: | | RPA32 |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Bochkareva, E. et al. J. Biol. Chem. 273:3932-3936 (1998) 2. Stigger, E. et al. J. Biol. Chem. 273:9337-9343 (1998) 3. Sibenaller, ZA. et al. Biochemistry. 37:12496-12506 (1998) 4. Dong, J. et al. Biochem.J. 337:311-317 (1999) 5. Bochkarev, A. et al. EMBO. J. 18:4498-4504 (1999) 6. Iftode, C. et al. Crit. Rev. Biochem. Mol. Biol. 34:141-180 (1999) 7. Treuner, K. et al. J. Biol. Chem. 274:15556-15561 (1999) |
| more information: | | company product webpage |
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