| CatalogCode: | NB100-343 |
| ProductName: | XRCC4 Antibody |
| Product Description: | Mouse Polyclonal anti-XRCC4 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant protein encoding an amino terminalfragment of human XRCC4 purified from E. coli. |
| Specificity: | This antibody is specific for XRCC4. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.05 ml Whole Mouse antisera. |
| Uses: | Western Blot: use at a dilution of 1:1000. Optimal working dilutions / concentrations should be determined by the end user.In Western blot, this antibody detects a band of approximately 46 kDa.
For IF, 1/50 in PBS:BSA, at least 30 min at room temperature. |
| Localization: | Nuclear |
| Background: | XRCC4 (X-ray cross complementation protein-4) is an essential component for DNA double strand break repair and V(D)J recombination. The XRCC4 gene encodes a 38kDa protein that is ubiquitously expressed and contains no sequence similarity to any previously described protein. Recent experiments have shown that XRCC4 directly interacts with DNA ligase IV implicating a possible role in DNA-end joining. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | PBS [pH7.4] |
| ListPrice: | 285 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-DNA double-strand break repair and V(D)J recombination protein XRCC4 antibody, anti-DNA-repair protein XRCC4 antibody, anti-X-ray repair complementing defective repair in Chinese hamster cells 4 antibody, anti-X-ray repair cross-complementing protein 4 antibody |
| ProteinTarget: | XRCC-4 |
| PackageSize: | 0.05 ml |
| GeneralRef: | Chen L et al. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem 275:26196-205 (2000). Li Z et al. The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination. Cell 83:1079-89 (1995). |