| CatalogCode: | NB100-332 |
| ProductName: | RPA32 Antibody |
| Product Description: | Rabbit Polyclonal anti-RPA32 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide, which represented a portion of C-terminus of human replication protein A2, 32 kDa subunit (LocusLink ID 6118). |
| Specificity: | NB 100-332 is specific for human RPA32 protein. |
| CrossReactivity: | NB 100-332 reacts with human. Other species have not been tested. Reactivity with mouse is expected based upon a single mismatch between the peptide used for this antibody production and mouse RPA32 at that site. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | Uses: NB 100-332 may be used for Western Blot and immunoprecipitation.Suggested working dilutions: *Western Blot - 1:500-1:2,500 (ECL)Immunohisto/cytochemistry - Not DeterminedImmunoprecipitation - 2 to 5 ug/mg lysate*The investigator should determine the optimal working dilution for a specific application. |
| Background: | Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| ListPrice: | 285 |
| NovusRef: | 1. Stewart, G.S. et al. CELL BIOLOGY: RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. PNAS. 104:16910-16915, Oct 2007 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-RPA2 antibody, anti-RPA32 antibody, anti-REPA2 antibody, anti-Replication protein A2 antibody, anti-32 kDA antibody, anti-RFA2 antibody, anti-RPA-p34 antibody |
| ProteinTarget: | RPA32 |
| PackageSize: | 0.1 ml |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |
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