| CatalogCode: | NB100-320 |
| ProductName: | FEN-1 Antibody |
| Product Description: | Rabbit Polyclonal anti-FEN-1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide, which represented a portion of human Flap Structure-specific Endonuclease 1 encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of the latter half of the N-domain as defined I Swiss-Prot entry P39748. |
| Specificity: | NB 100-320 is specific for human FEN-1 protein. |
| CrossReactivity: | NB 100-320 reacts with human. Other species have not been tested. Reactivity with mouse FEN-1 is likely owing to complete identity between ortholog and the peptide used as the immunogen and Xenopus FEN-1 has 1 mismatch with the peptide used as the immunogen. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | NB 100-320 may be used for Western Blot and immunoprecipitation.Suggested working dilutions: *WB - 1:5,000-1:30,000 (ECL)IHC / ICC - Not DeterminedIP - 4 to 10 ug/ mg of lysate*The investigator should determine the optimal working dilution for a specific application. |
| Background: | FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| ListPrice: | 285 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Flap endonuclease 1 antibody, anti-flap structure specific endonuclease 1 antibody, anti-Maturation factor 1 antibody, anti-MF1 antibody, anti-RAD2 (S. pombe) homolog antibody, anti-fen1 antibody |
| ProteinTarget: | FEN-1 |
| PackageSize: | 0.1 ml |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |