| CatalogCode: | NB100-310SS |
| ProductName: | HIF Prolyl Hydroxylase 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-HIF Prolyl Hydroxylase 1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptides made to a C-terminus portion of the human EGL9 homolog 2. [LocusLink ID 111298] |
| Specificity: | This antibody is specific for HPH-3. |
| CrossReactivity: | Human, rat and mouse. Other species have not been tested. |
| Packaging: | 0.025 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody may be used for Western blotting where a band is seen at 45-50 kDa, representing HPH-3. *Optimal dilutions should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Control: | HeLa and MEF extracts |
| Background: | HIF prolyl hydroxylase 1 (HPH-3)is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate [pH 7-8] |
| ListPrice: | 100 |
| NovusRef: | 1. Li N., et al. Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys. Am J Physiol Renal Physiol. 292: F207 - F216, 2007. 2. Groenman, F., Rutter, M., Caniggia, I., Tibboel, D., and Post, M. (2007) Hypoxia-inducible Factors in the First Trimester Human Lung, 55, 355-363. 3. Baek, J., et al. OS-9 Interacts with Hypoxia-Inducible Factor 1 alpha and Prolyl Hydroxylases to Promote Oxygen-Dependent Degradation of HIF-1alpha. Molecular Cell. 17: 503-512, 2005. 4. Asikainen, T.M., et al. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. PNAS. 102(29): 10212-10217, 2005. (Western blot, human) 5. McMahon, S., et al. PROTEIN SYNTHESIS, POST-TRANSLATION MODIFICATION, AND DEGRADATION: TGF beta 1 induces HIF-1 stabilization through selective inhition of PHD2 expression. J. Biol. Chem., 10.1074/jbc.M604507200, 2006. 6. Ietta, F., et al. Dynamic HIF-1alpha regulation during human placental development. Biol. Reprod. 2006. 7. Takeda K, Aguila HL, Parikh NS, et al. Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins. Blood. March 15, 2008;111(6):3229-35. 8. Mikhaylova O, Ignacak ML, Barankiewicz TJ, et al. The von Hippel-Lindau Tumor Suppressor Protein and Egl-9-Type Proline Hydroxylases Regulate the Large Subunit of RNA Polymerase II in Response to Oxidative Stress. Mol Cell Biol. April 15, 2008;28(8):2701-17. 9. Lee K, Lynd JD, O'Reilly S, Kiupel M, McCormick JJ, LaPres JJ. The Biphasic Role of the Hypoxia-Inducible Factor Prolyl-4-Hydroxylase, PHD2, in Modulating Tumor-Forming Potential. Mol Cancer Res. May 1, 2008;6(5):829-42. |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Rt, Mu, Rt |
| ALTnames: | anti-EGL nine (C.elegans) homolog 2 antibody; anti-EGLN2 antibody; anti-estrogen-induced tag 6 antibody; anti-HIF prolyl hydroxylase 1 antibody; anti-HIF-P4H 1 antibody; anti-P4H1 antibody; anti-prolyl hydroxylase domain-containing protein 1 antibody |
| ProteinTarget: | HIF Prolyl Hydroxylase 1 (aka HPH-3, PHD1 and EGLN2) |
| PackageSize: | 0.025 ml |
| GeneralRef: | 1. Bruick, RK, McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294: 1337-1340 (2001) 2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21): 13459-13464 (2002) 3. Maxwell, PH, Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13: 29-37 (2002) |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please enquire availability. |
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