| CatalogCode: | NB100-304SS |
| ProductName: | 53BP1 Antibody |
| Product Description: | Rabbit Polyclonal anti-53BP1 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide, which represents a portion of human tumor protein p53 binding protein 1 encoded in part by exon 11 and 12 (LocusLink ID 7158). |
| Specificity: | NB 100-304 is specific for human 53BP1 protein. |
| CrossReactivity: | NB 100-304 reacts with human and mouse proteins. Other species have not been tested. |
| Packaging: | 0.025 ml Rabbit antisera. |
| Uses: | NB 100-304 may be used for Western Blot where a band at ~220 kD is seen on SDS-PAGE. Irradiated lysates demonstrate a gel shift. |
| Localization: | Nuclear. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. |
| Control: | U205, 293T, MO59K cell lysates (human) and MEF lysates (mouse) |
| Background: | P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability. |
| ProductRef: | 1. Riballo, et al. Mol Cell 16(5): 715-724 (2004) |
| Storage: | Store at 4C. Do not freeze. |
| Host_Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate [pH7-8] |
| ListPrice: | 100 |
| NovusRef: | 1. Konishi A, de Lange T. Cell cycle control of telomere protection and NHEJ revealed by a ts mutation in the DNA-binding domain of TRF2. Genes & Dev. May 1, 2008;22(9):1221-30. |
| AppSummary: | IF, WB, ICC |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-53BP1 antibody; anti-53BP-1 antibody; anti-TP53BP1 antibody; anti-Tumor Protein p53 Binding Protein 1 antibody |
| PackageSize: | 0.025 ml |
| NotesMain: | Blocking peptide is available or can be made for this item. Lead time is 3 days to 4 weeks. Price is $ 150/0.25 ml (or 50 ug). Please inquire availability. |